PDBe 2a1a

X-ray diffraction
2.8Å resolution

PKR kinase domain-eIF2alpha Complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Eukaryotic translation initiation factor 2 subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 175 amino acids
Theoretical weight: 20.55 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P20459 (Residues: 3-176; Coverage: 57%)
Gene names: J1429, SUI2, TIF211, YJR007W
Sequence domains: S1 RNA binding domain
Structure domains:
Interferon-induced, double-stranded RNA-activated protein kinase Chain: B
Molecule details ›
Chain: B
Length: 284 amino acids
Theoretical weight: 32.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P19525 (Residues: 258-551; Coverage: 51%)
Gene names: EIF2AK2, PKR, PRKR
Structure domains:

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P3221
Unit cell:
a: 84.3Å b: 84.3Å c: 165.4Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.213 0.207 0.268
Expression system: Escherichia coli