PDBe 2a11

X-ray diffraction
2.1Å resolution

Crystal Structure of Nuclease Domain of Ribonuclase III from Mycobacterium Tuberculosis

Released:
Source organism: Mycobacterium tuberculosis
Primary publication:
Structure of the nuclease domain of ribonuclease III from M. tuberculosis at 2.1 A.
Protein Sci. 14 2744-50 (2005)
PMID: 16155207

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage to 5'-phosphomonoester. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease 3 Chain: A
Molecule details ›
Chain: A
Length: 242 amino acids
Theoretical weight: 25.56 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WH03 (Residues: 1-240; Coverage: 100%)
Gene names: MTCY338.14c, Rv2925c, rnc
Sequence domains:
Structure domains: Ribonuclease iii, N-terminal Endonuclease Domain; Chain A

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1, null
Spacegroup: P43212
Unit cell:
a: 72.589Å b: 72.589Å c: 96.013Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.286 0.258 0.286
Expression system: Escherichia coli