PDBe 2zo2

X-ray diffraction
3.09Å resolution

Mouse NP95 SRA domain non-specific DNA complex

Released:

Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Structure domain:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
homo dimer
monomeric
hetero hexamer
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
E3 ubiquitin-protein ligase UHRF1 Chain: B
Molecule details ›
Chain: B
Length: 212 amino acids
Theoretical weight: 23.92 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8VDF2 (Residues: 419-628; Coverage: 27%)
Gene names: Np95, Uhrf1
Sequence domains: SAD/SRA domain
Structure domains: SRA-YDG
DNA (5'-D(*DAP*DAP*DCP*DTP*DGP*DCP*DGP*DCP*DAP*DGP*DTP*DT)-3') Chains: D, E
Molecule details ›
Chains: D, E
Length: 12 nucleotides
Theoretical weight: 3.66 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P6122
Unit cell:
a: 81.551Å b: 81.551Å c: 182.165Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.232 0.232 0.291
Expression systems:
  • Escherichia coli
  • Not provided