› 2ze6

X-ray diffraction
2.1Å resolution

Crystal Structure of adenosine phosphate-isopentenyltransferase complexed with substrate analog, DMASPP

Released:
DOI: 10.2210/pdb2ze6/pdb
PDB entry 2ze6 coloured by chain, front view.
PDB entry 2ze6 coloured by chain, side view.
PDB entry 2ze6 coloured by chain, top view.
Source organism: Agrobacterium tumefaciens
Primary publication:
Structural insight into the reaction mechanism and evolution of cytokinin biosynthesis.
Sugawara H, Ueda N, Kojima M, Makita N, Yamaya T, Sakakibara H
Proc Natl Acad Sci U S A 105 2734-9 (2008)
PMID: 18258747

Function and Biology Details

Reaction catalysed: 
Prenyl diphosphate + AMP = diphosphate + N(6)-prenyladenosine 5'-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157721 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenylate dimethylallyltransferase Chain: A
Molecule details ›
Chain: A
Length: 253 amino acids
Theoretical weight: 28.84 KDa
Source organism: Agrobacterium tumefaciens
Expression system: Escherichia coli
UniProt:
  • Canonical: P58758 (Residues: 1-243; Coverage: 100%)
Gene names: AGR_pTi_290, Atu6164, tzs
Sequence domains: Isopentenyl transferase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand DST 1 x DST
Ligand AMP 1 x AMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL45PX
Spacegroup: P42212
Unit cell:
a: 96.417Å b: 96.417Å c: 65.068Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.196 0.264
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Cytokinin action in plant development.
Werner et al. (2009)
4 more

2 mentions without citation

Biochemical and Structural Aspects of Cytokinin Biosynthesis and Degradation in Bacteria.
Frébortová et al. (2021)
1 more