PDBe 2z2w

X-ray diffraction
2.22Å resolution

Human Wee1 kinase complexed with inhibitor PF0335770

Released:
Source organism: Homo sapiens
Entry authors: Squire CJ, Baker EN

Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Wee1-like protein kinase Chain: A
Molecule details ›
Chain: A
Length: 285 amino acids
Theoretical weight: 32.18 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P30291 (Residues: 291-575; Coverage: 44%)
Gene name: WEE1
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

3 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P41212
Unit cell:
a: 69.747Å b: 69.747Å c: 157.215Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.215 0.271
Expression system: Spodoptera frugiperda