PDBe 2yjt

X-ray diffraction
2.9Å resolution

Crystal structure of E. coli DEAD-box protein SrmB bound to regulator of ribonuclease activity A (RraA)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ATP-dependent RNA helicase SrmB Chain: D
Molecule details ›
Chain: D
Length: 170 amino acids
Theoretical weight: 19.56 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P21507 (Residues: 219-388; Coverage: 38%)
Gene names: JW2560, b2576, rbaB, rhlA, srmB
Sequence domains: Helicase conserved C-terminal domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Regulator of ribonuclease activity A Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 161 amino acids
Theoretical weight: 17.37 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0A8R0 (Residues: 1-161; Coverage: 100%)
Gene names: JW3900, b3929, menG, rraA, yiiV
Sequence domains: Aldolase/RraA
Structure domains: RraA-like

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P3221
Unit cell:
a: 73.39Å b: 73.39Å c: 222.891Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.195 0.192 0.257
Expression system: Escherichia coli BL21