PDBe 2xwt

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURE OF THE TSH RECEPTOR IN COMPLEX WITH A BLOCKING TYPE TSHR AUTOANTIBODY

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
THYROID BLOCKING HUMAN AUTOANTIBODY K1-70 HEAVY CHAIN Chain: A
Molecule details ›
Chain: A
Length: 221 amino acids
Theoretical weight: 23.64 KDa
Source organism: Homo sapiens
Expression system: hybrid
Structure domains: Immunoglobulins
THYROID BLOCKING HUMAN AUTOANTIBODY K1-70 LIGHT CHAIN Chain: B
Molecule details ›
Chain: B
Length: 214 amino acids
Theoretical weight: 22.6 KDa
Source organism: Homo sapiens
Expression system: hybrid
Structure domains: Immunoglobulins
Thyrotropin receptor Chain: C
Molecule details ›
Chain: C
Length: 239 amino acids
Theoretical weight: 26.94 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P16473 (Residues: 22-260; Coverage: 32%)
Gene names: LGR3, TSHR
Sequence domains: BspA type Leucine rich repeat region (6 copies)
Structure domains: Ribonuclease Inhibitor

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 83.02Å b: 89.3Å c: 101.27Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.177 0.227
Expression systems:
  • hybrid
  • Trichoplusia ni