PDBe 2xrw

X-ray diffraction
1.33Å resolution

Linear binding motifs for JNK and for calcineurin antagonistically control the nuclear shuttling of NFAT4

Released:

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mitogen-activated protein kinase 8 Chain: A
Molecule details ›
Chain: A
Length: 371 amino acids
Theoretical weight: 42.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P45983 (Residues: 2-364; Coverage: 85%)
  • Best match: P45983-3 (Residues: 2-364)
Gene names: JNK1, MAPK8, PRKM8, SAPK1, SAPK1C
Sequence domains: Protein kinase domain
Structure domains:
Nuclear factor of activated T-cells, cytoplasmic 3 Chain: B
Molecule details ›
Chain: B
Length: 14 amino acids
Theoretical weight: 1.74 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q12968 (Residues: 141-154; Coverage: 1%)
Gene names: NFAT4, NFATC3

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 46.23Å b: 94.13Å c: 47.84Å
α: 90° β: 110.74° γ: 90°
R-values:
R R work R free
0.166 0.165 0.187
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided