PDBe 2xac

X-ray diffraction
2.71Å resolution

Structural Insights into the Binding of VEGF-B by VEGFR-1D2: Recognition and Specificity

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Vascular endothelial growth factor B Chains: A, B
Molecule details ›
Chains: A, B
Length: 99 amino acids
Theoretical weight: 11.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: NEW P49765 (Residues: 31-129; Coverage: 53%)
Gene names: VEGFB, VRF
Sequence domains: PDGF/VEGF domain
Structure domains: Cystine-knot cytokines
Vascular endothelial growth factor receptor 1 Chains: C, X
Molecule details ›
Chains: C, X
Length: 98 amino acids
Theoretical weight: 11.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: NEW P17948 (Residues: 129-226; Coverage: 8%)
Gene names: FLT, FLT1, FRT, VEGFR1
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P21
Unit cell:
a: 39.212Å b: 65.526Å c: 82.824Å
α: 90° β: 90.26° γ: 90°
R-values:
R R work R free
0.286 0.282 0.364
Expression systems:
  • Escherichia coli
  • Escherichia coli BL21(DE3)