PDBe 2x4x

X-ray diffraction
1.85Å resolution

Molecular basis of Histone H3K36me3 recognition by the PWWP domain of BRPF1.

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Peregrin Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 132 amino acids
Theoretical weight: 15.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P55201 (Residues: 1076-1205; Coverage: 11%)
Gene names: BR140, BRPF1
Sequence domains: PWWP domain
Structure domains: SH3 type barrels.
Histone H3.2 Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 21 amino acids
Theoretical weight: 2.33 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q71DI3 (Residues: 23-43; Coverage: 15%)
Gene names: H3F2, H3FM, HIST2H3A, HIST2H3C, HIST2H3D

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21
Unit cell:
a: 38.437Å b: 102.941Å c: 88.654Å
α: 90° β: 100.33° γ: 90°
R-values:
R R work R free
0.199 0.197 0.239
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided