PDBe 2x4s

X-ray diffraction
2.55Å resolution

Crystal structure of MHC CLass I HLA-A2.1 bound to a peptide representing the epitope of the H5N1 (Avian Flu) Nucleoprotein

Source organisms:
Primary publication:
UV-induced ligand exchange in MHC class I protein crystals.
J. Am. Chem. Soc. 131 12298-304 (2009)
PMID: 19655750

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
HLA class I histocompatibility antigen, A-2 alpha chain Chains: A, D
Molecule details ›
Chains: A, D
Length: 275 amino acids
Theoretical weight: 31.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P01892 (Residues: 25-299; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, E
Molecule details ›
Chains: B, E
Length: 100 amino acids
Theoretical weight: 11.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Nucleoprotein Chains: C, F
Molecule details ›
Chains: C, F
Length: 9 amino acids
Theoretical weight: 993 Da
Source organism: Influenza A virus
Expression system: Not provided
  • Canonical: Q33CG5 (Residues: 6-14; Coverage: 7%)
Gene name: NP

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 62.377Å b: 84.112Å c: 80.035Å
α: 90° β: 90.97° γ: 90°
R R work R free
0.182 0.178 0.248
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli
  • Not provided