PDBe 2wpn

X-ray diffraction
2.04Å resolution

Structure of the oxidised, as-isolated NiFeSe hydrogenase from D. vulgaris Hildenborough

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Periplasmic [NiFeSe] hydrogenase, small subunit Chain: A
Molecule details ›
Chain: A
Length: 317 amino acids
Theoretical weight: 33.94 KDa
Source organism: Desulfovibrio vulgaris str. Hildenborough
UniProt:
  • Canonical: Q72AS4 (Residues: 1-317; Coverage: 100%)
Gene names: DVU_1917, hysB
Sequence domains:
Structure domains:
Periplasmic [NiFeSe] hydrogenase, large subunit, selenocysteine-containing Chain: B
Molecule details ›
Chain: B
Length: 495 amino acids
Theoretical weight: 54.45 KDa
Source organism: Desulfovibrio vulgaris str. Hildenborough
UniProt:
  • Canonical: Q72AS3 (Residues: 1-495; Coverage: 97%)
Gene names: DVU_1918, hysA
Sequence domains: Nickel-dependent hydrogenase
Structure domains: Cytochrome-c3 Hydrogenase, chain B

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21
Unit cell:
a: 60.596Å b: 91.223Å c: 66.751Å
α: 90° β: 101.73° γ: 90°
R-values:
R R work R free
0.147 0.144 0.201