PDBe 2wk3

X-ray diffraction
2.59Å resolution

Crystal structure of human insulin-degrading enzyme in complex with amyloid-beta (1-42)

Released:

Function and Biology Details

Reaction catalysed:
Degradation of insulin, glucagon and other polypeptides. No action on proteins. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Beta-amyloid protein 42 Chains: C, D
Molecule details ›
Chains: C, D
Length: 42 amino acids
Theoretical weight: 4.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P05067 (Residues: 672-713; Coverage: 6%)
Gene names: A4, AD1, APP
Sequence domains: Beta-amyloid peptide (beta-APP)
Insulin-degrading enzyme Chains: A, B
Molecule details ›
Chains: A, B
Length: 1019 amino acids
Theoretical weight: 117.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P14735 (Residues: 1-1019; Coverage: 100%)
Gene name: IDE
Sequence domains:
Structure domains: Cytochrome Bc1 Complex; Chain A, domain 1

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P65
Unit cell:
a: 261.628Å b: 261.628Å c: 90.675Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.189 0.187 0.232
Expression system: Escherichia coli BL21(DE3)