PDBe 2wh0

X-ray diffraction
2.25Å resolution

Recognition of an intrachain tandem 14-3-3 binding site within protein kinase C epsilon

Released:

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein zeta/delta Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 245 amino acids
Theoretical weight: 27.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63104 (Residues: 1-245; Coverage: 100%)
Gene name: YWHAZ
Sequence domains: 14-3-3 protein
Structure domains: 14-3-3 domain
Protein kinase C epsilon type Chains: Q, R
Molecule details ›
Chains: Q, R
Length: 31 amino acids
Theoretical weight: 3.73 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q02156 (Residues: 342-372; Coverage: 4%)
Gene names: PKCE, PRKCE

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 71.13Å b: 78.16Å c: 108.52Å
α: 90° β: 90.1° γ: 90°
R-values:
R R work R free
0.183 0.181 0.235
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided