PDBe 2vrw

X-ray diffraction
1.85Å resolution

Critical structural role for the PH and C1 domains of the Vav1 exchange factor

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Proto-oncogene vav Chain: B
Molecule details ›
Chain: B
Length: 406 amino acids
Theoretical weight: 47.5 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P27870 (Residues: 170-575; Coverage: 48%)
Gene names: Vav, Vav1
Sequence domains:
Structure domains:
Ras-related C3 botulinum toxin substrate 1 Chain: A
Molecule details ›
Chain: A
Length: 184 amino acids
Theoretical weight: 20.46 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P63000 (Residues: 1-184; Coverage: 96%)
Gene names: MIG5, RAC1, TC25
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX10.1
Spacegroup: C2
Unit cell:
a: 122.763Å b: 62.143Å c: 103.281Å
α: 90° β: 118.8° γ: 90°
R-values:
R R work R free
0.205 0.203 0.251
Expression system: Escherichia coli BL21