PDBe 2vmk

X-ray diffraction
3.3Å resolution

Crystal Structure of E. coli RNase E Apoprotein - Catalytic Domain

Released:

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage of single-stranded RNA in A- and U-rich regions. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease E Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 515 amino acids
Theoretical weight: 57.91 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P21513 (Residues: 1-515; Coverage: 49%)
Gene names: JW1071, ams, b1084, hmp1, rne
Sequence domains:

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P1
Unit cell:
a: 73.241Å b: 75.571Å c: 109.37Å
α: 94.95° β: 102.03° γ: 91.77°
R-values:
R R work R free
0.268 0.267 0.293
Expression system: Escherichia coli BL21(DE3)