PDBe 2vlk

X-ray diffraction
2.5Å resolution

The Structural Dynamics and Energetics of an Immunodominant T-cell Receptor are Programmed by its Vbeta Domain

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
HLA class I histocompatibility antigen, A-2 alpha chain Chain: A
Molecule details ›
Chain: A
Length: 276 amino acids
Theoretical weight: 31.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01892 (Residues: 25-300; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
FLU MATRIX PEPTIDE Chain: C
Molecule details ›
Chain: C
Length: 9 amino acids
Theoretical weight: 966 Da
Source organism: unidentified influenza virus
Expression system: Not provided
JM22 TCR ALPHA CHAIN Chain: D
Molecule details ›
Chain: D
Length: 201 amino acids
Theoretical weight: 22.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
JM22 TCR BETA CHAIN Chain: E
Molecule details ›
Chain: E
Length: 244 amino acids
Theoretical weight: 27.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: C2
Unit cell:
a: 210.8Å b: 47.892Å c: 112.819Å
α: 90° β: 112.34° γ: 90°
R-values:
R R work R free
0.221 0.218 0.288
Expression systems:
  • Escherichia coli
  • Not provided