PDBe 2v89

X-ray diffraction
1.1Å resolution

Crystal structure of RAG2-PHD finger in complex with H3K4me3 peptide at 1.1A resolution

Released:

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
V(D)J recombination-activating protein 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 82 amino acids
Theoretical weight: 9.36 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: NEW P21784 (Residues: 414-487; Coverage: 14%)
Gene names: Rag-2, Rag2
Structure domains: Double Stranded RNA Binding Domain
Histone H3.1 Chains: D, E
Molecule details ›
Chains: D, E
Length: 10 amino acids
Theoretical weight: 1.09 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: NEW P68431 (Residues: 2-11; Coverage: 7%)
Gene names: H3FA, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 75.978Å b: 46.807Å c: 56.808Å
α: 90° β: 106.78° γ: 90°
R-values:
R R work R free
0.123 not available 0.155
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided