PDBe 2v1y

X-ray diffraction
2.4Å resolution

Structure of a phosphoinositide 3-kinase alpha adaptor-binding domain (ABD) in a complex with the iSH2 domain from p85 alpha

Released:

Function and Biology Details

Reactions catalysed:
ATP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate = ADP + 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform Chain: A
Molecule details ›
Chain: A
Length: 108 amino acids
Theoretical weight: 12.82 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: NEW P32871 (Residues: 1-108; Coverage: 10%)
Gene name: PIK3CA
Sequence domains: PI3-kinase family, p85-binding domain
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
Phosphatidylinositol 3-kinase regulatory subunit alpha Chain: B
Molecule details ›
Chain: B
Length: 170 amino acids
Theoretical weight: 21.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: NEW P27986 (Residues: 431-600; Coverage: 24%)
Gene names: GRB1, PIK3R1
Sequence domains: Phosphatidylinositol 3-kinase regulatory subunit P85 inter-SH2 domain

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 58.799Å b: 62Å c: 74.654Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.236 0.233 0.292
Expression system: Escherichia coli