PDBe 2uuf

X-ray diffraction
1.26Å resolution

Thrombin-hirugen binary complex at 1.26A resolution

Released:
Source organisms:
Primary publication:
The determination of protonation states in proteins.
Acta Crystallogr. D Biol. Crystallogr. 63 906-22 (2007)
PMID: 17642517

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Thrombin light chain Chain: A
Molecule details ›
Chain: A
Length: 36 amino acids
Theoretical weight: 4.1 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P00734 (Residues: 328-363; Coverage: 6%)
Gene name: F2
Thrombin heavy chain Chain: B
Molecule details ›
Chain: B
Length: 259 amino acids
Theoretical weight: 29.78 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P00734 (Residues: 364-622; Coverage: 43%)
Gene name: F2
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Hirudin variant-1 Chain: H
Molecule details ›
Chain: H
Length: 10 amino acids
Theoretical weight: 1.36 KDa
Source organism: Hirudo medicinalis
Expression system: Not provided
UniProt:
  • Canonical: P01050 (Residues: 55-64; Coverage: 15%)

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX10.1
Spacegroup: C2
Unit cell:
a: 70.275Å b: 71.29Å c: 72.037Å
α: 90° β: 99.9° γ: 90°
R-values:
R R work R free
0.146 not available 0.194
Expression system: Not provided