PDBe 2rs9

Solution NMR

Solution structure of the bromodomain of human BRPF1 in complex with histone H4K5ac peptide

Released:
Source organism: Homo sapiens
Entry authors: Qin X, Nagashima T, Umehara T, Hayashi F, Yokoyama S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Histone H4 Chain: A
Molecule details ›
Chain: A
Length: 10 amino acids
Theoretical weight: 960 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: NEW P62805 (Residues: 2-11; Coverage: 10%)
Gene names: H4/A, H4/B, H4/C, H4/D, H4/E, H4/G, H4/H, H4/I, H4/J, H4/K, H4/M, H4/N, H4/O, H4F2, H4FA, H4FB, H4FC, H4FD, H4FE, H4FG, H4FH, H4FI, H4FJ, H4FK, H4FM, H4FN, H4FO, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4A, HIST2H4B, HIST4H4
Peregrin Chain: B
Molecule details ›
Chain: B
Length: 121 amino acids
Theoretical weight: 13.78 KDa
Source organism: Homo sapiens
Expression system: CELL-FREE SYNTHESIS
UniProt:
  • Canonical: NEW P55201 (Residues: 633-740; Coverage: 9%)
Gene names: BR140, BRPF1
Sequence domains: Bromodomain
Structure domains: Bromodomain-like

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 92%
Refinement method: torsion angle dynamics, molecular dynamics
Chemical shifts: BMR11463  
Expression system: CELL-FREE SYNTHESIS