PDBe 2rfx

X-ray diffraction
2.5Å resolution

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
HLA class I histocompatibility antigen, B-57 alpha chain Chain: A
Molecule details ›
Chain: A
Length: 275 amino acids
Theoretical weight: 31.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P18465 (Residues: 25-299; Coverage: 81%)
Gene names: HLA-B, HLAB
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 99 amino acids
Theoretical weight: 11.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Immunoglobulin kappa constant Chain: C
Molecule details ›
Chain: C
Length: 9 amino acids
Theoretical weight: 966 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01834 (Residues: 94-102; Coverage: 8%)
Gene name: IGKC

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P21
Unit cell:
a: 64.844Å b: 49.029Å c: 70.666Å
α: 90° β: 100.7° γ: 90°
R-values:
R R work R free
0.212 0.209 0.268
Expression systems:
  • Escherichia coli BL21
  • Not provided