PDBe 2rf9

X-ray diffraction
3.5Å resolution

Crystal structure of the complex between the EGFR kinase domain and a Mig6 peptide

Released:

Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Epidermal growth factor receptor Chains: A, B
Molecule details ›
Chains: A, B
Length: 330 amino acids
Theoretical weight: 37.56 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P00533 (Residues: 696-1022; Coverage: 28%)
Gene names: EGFR, ERBB, ERBB1, HER1
Sequence domains: Protein tyrosine kinase
Structure domains:
ERBB receptor feedback inhibitor 1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 65 amino acids
Theoretical weight: 6.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UJM3 (Residues: 315-374; Coverage: 13%)
Gene names: ERRFI1, MIG6
Sequence domains: EGFR receptor inhibitor Mig-6

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: C2
Unit cell:
a: 188.762Å b: 42.65Å c: 99.726Å
α: 90° β: 109.12° γ: 90°
R-values:
R R work R free
0.272 0.272 0.329
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli BL21(DE3)