PDBe 2qna

X-ray diffraction
2.84Å resolution

Crystal structure of human Importin-beta (127-876) in complex with the IBB-domain of Snurportin1 (1-65)

Released:
Source organism: Homo sapiens

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Importin subunit beta-1 Chain: A
Molecule details ›
Chain: A
Length: 762 amino acids
Theoretical weight: 84.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q14974 (Residues: 127-875; Coverage: 86%)
Gene names: KPNB1, NTF97
Sequence domains: Armadillo/beta-catenin-like repeat
Structure domains: Leucine-rich Repeat Variant
Snurportin-1 Chain: B
Molecule details ›
Chain: B
Length: 66 amino acids
Theoretical weight: 7.68 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O95149 (Residues: 1-66; Coverage: 18%)
Gene names: RNUT1, SNUPN, SPN1
Sequence domains: Snurportin1

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P212121
Unit cell:
a: 64.22Å b: 79.07Å c: 208.46Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.233 0.282
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli