PDBe 2qlv

X-ray diffraction
2.6Å resolution

Crystal structure of the heterotrimer core of the S. cerevisiae AMPK homolog SNF1

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Carbon catabolite-derepressing protein kinase Chains: A, D
Molecule details ›
Chains: A, D
Length: 171 amino acids
Theoretical weight: 19.6 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P06782 (Residues: 460-630; Coverage: 27%)
Gene names: CAT1, CCR1, D8035.20, GLC2, PAS14, SNF1, YDR477W
Sequence domains: Adenylate sensor of SNF1-like protein kinase
Structure domains: Kinase associated domain 1, KA1
SNF1 protein kinase subunit beta-2 Chains: B, E
Molecule details ›
Chains: B, E
Length: 252 amino acids
Theoretical weight: 28.96 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P34164 (Residues: 161-412; Coverage: 61%)
Gene names: G1155, SIP2, SPM2, YGL208W
Sequence domains:
Structure domains:
5'-AMP-activated protein kinase subunit gamma Chains: C, F
Molecule details ›
Chains: C, F
Length: 315 amino acids
Theoretical weight: 35.63 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P12904 (Residues: 7-321; Coverage: 98%)
Gene names: CAT3, SCI1, SNF4, YGL115W
Sequence domains: CBS domain
Structure domains: CBS-domain

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2
Unit cell:
a: 112.33Å b: 81.84Å c: 174.74Å
α: 90° β: 102.22° γ: 90°
R-values:
R R work R free
0.239 0.239 0.299
Expression system: Escherichia coli