PDBe 2q8i

X-ray diffraction
2.6Å resolution

Pyruvate dehydrogenase kinase isoform 3 in complex with antitumor drug radicicol

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 3, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 419 amino acids
Theoretical weight: 48.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q15120 (Residues: 9-406; Coverage: 98%)
Gene names: PDHK3, PDK3
Sequence domains:
Structure domains:
Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial Chain: B
Molecule details ›
Chain: B
Length: 128 amino acids
Theoretical weight: 14.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P10515 (Residues: 212-319; Coverage: 17%)
Gene names: DLAT, DLTA
Sequence domains: Biotin-requiring enzyme
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P6522
Unit cell:
a: 120.872Å b: 120.872Å c: 239.637Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.205 0.238
Expression system: Escherichia coli BL21