PDBe 2q8e

X-ray diffraction
2.05Å resolution

Specificity and Mechanism of JMJD2A, a Trimethyllysine-Specific Histone Demethylase

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
histone 3 peptide Chains: F, G
Molecule details ›
Chains: F, G
Length: 16 amino acids
Theoretical weight: 1.8 KDa
Source organism: Synthetic construct
Expression system: Not provided
Lysine-specific demethylase 4A Chains: A, B
Molecule details ›
Chains: A, B
Length: 352 amino acids
Theoretical weight: 40.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O75164 (Residues: 1-350; Coverage: 33%)
Gene names: JHDM3A, JMJD2, JMJD2A, KDM4A, KIAA0677
Sequence domains:

Ligands and Environments

3 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P21212
Unit cell:
a: 100.294Å b: 148.964Å c: 56.81Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.213 0.26
Expression systems:
  • Not provided
  • Escherichia coli BL21