› 2q0x

X-ray diffraction
2.2Å resolution

Alpha/Beta hydrolase fold protein of unknown function

Released:
DOI: 10.2210/pdb2q0x/pdb
PDB entry 2q0x coloured by chain, front view.
PDB entry 2q0x coloured by chain, side view.
PDB entry 2q0x coloured by chain, top view.
Source organism: Trypanosoma brucei
Primary publication:
Structure of a Trypanosoma brucei alpha/beta-hydrolase fold protein with unknown function.
Merritt EA, Holmes M, Buckner FS, Van Voorhis WC, Quartly E, Phizicky EM, Lauricella A, Luft J, DeTitta G, Neely H, Zucker F, Hol WG
Acta Crystallogr Sect F Struct Biol Cryst Commun 64 474-8 (2008)
PMID: 18540054

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174387 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Paraflagellar rod component Chains: A, B
Molecule details ›
Chains: A, B
Length: 335 amino acids
Theoretical weight: 37.36 KDa
Source organism: Trypanosoma brucei
Expression system: Escherichia coli
UniProt:
  • Canonical: Q389W3 (Residues: 1-331; Coverage: 100%)
Gene name: Tb10.6k15.0140
Sequence domains: Protein of unknown function (DUF1749)
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P3121
Unit cell:
a: 63.556Å b: 63.556Å c: 303.186Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.208 0.206 0.25
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Trypanosoma brucei Acyl-Protein Thioesterase-like (TbAPT-L) Is a Lipase with Esterase Activity for Short and Medium-Chain Fatty Acids but Has No Depalmitoylation Activity.
Brown et al. (2022)
1 more

2 mentions without citation

Lessons from high-throughput protein crystallization screening: 10 years of practical experience.
Luft et al. (2011)
1 more