PDBe 2pq9

X-ray diffraction
1.6Å resolution

E. coli EPSPS liganded with (R)-difluoromethyl tetrahedral reaction intermediate analog

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-phosphoshikimate 1-carboxyvinyltransferase Chain: A
Molecule details ›
Chain: A
Length: 427 amino acids
Theoretical weight: 46.14 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A6D3 (Residues: 1-427; Coverage: 100%)
Gene names: JW0891, aroA, b0908
Sequence domains: EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)
Structure domains: Enolpyruvate transferase domain

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P212121
Unit cell:
a: 58.157Å b: 85.101Å c: 87.644Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.166 0.191
Expression system: Escherichia coli BL21(DE3)