› 2pnm

X-ray diffraction
2.3Å resolution

Crystal Structure of VP4 protease from infectious pancreatic necrosis virus (IPNV) in space group P6122

Released:
DOI: 10.2210/pdb2pnm/pdb
PDB entry 2pnm coloured by chain, front view.
PDB entry 2pnm coloured by chain, side view.
PDB entry 2pnm coloured by chain, top view.
Source organism: Infectious pancreatic necrosis virus - Sp
Primary publication:
Crystal structure of the VP4 protease from infectious pancreatic necrosis virus reveals the acyl-enzyme complex for an intermolecular self-cleavage reaction.
Lee J, Feldman AR, Delmas B, Paetzel M
J Biol Chem 282 24928-37 (2007)
PMID: 17553791

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106741 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PP Chain: A
Molecule details ›
Chain: A
Length: 193 amino acids
Theoretical weight: 20.59 KDa
Source organism: Infectious pancreatic necrosis virus - Sp
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A1XQR0 (Residues: 524-716; Coverage: 20%)
Sequence domains: Birnavirus VP4 protein
Structure domains: Ribosomal Protein S5; domain 2

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 3 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P6122
Unit cell:
a: 76.953Å b: 76.953Å c: 136.347Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.2 0.182 0.235
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

The penicillin-binding proteins: structure and role in peptidoglycan biosynthesis.
Sauvage et al. (2008)
1 more

4 mentions without citation

Analyses of the radiation of birnaviruses from diverse host phyla and of their evolutionary affinities with other double-stranded RNA and positive strand RNA viruses using robust structure-based multiple sequence alignments and advanced phylogenetic methods.
Gibrat et al. (2013)
3 more