PDBe 2pme

X-ray diffraction
2.9Å resolution

The Apo crystal Structure of the glycyl-tRNA synthetase

Released:
Source organism: Homo sapiens

Function and Biology Details

Reactions catalysed:
P(1),P(4)-bis(5'-guanosyl) tetraphosphate + H(2)O = GTP + GMP
ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly)
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycine--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 693 amino acids
Theoretical weight: 78.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P41250 (Residues: 55-739; Coverage: 93%)
Gene name: GARS
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P43212
Unit cell:
a: 91.742Å b: 91.742Å c: 247.176Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.23 0.271
Expression system: Escherichia coli BL21(DE3)