PDBe 2pi0

X-ray diffraction
2.31Å resolution

Crystal Structure of IRF-3 bound to the PRDIII-I regulatory element of the human interferon-B enhancer

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Interferon regulatory factor 3 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 116 amino acids
Theoretical weight: 13.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14653 (Residues: 1-113; Coverage: 27%)
Gene name: IRF3
Sequence domains: Interferon regulatory factor transcription factor
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
PRDIII-I region of human interferon-B promoter strand 1 Chain: E
Molecule details ›
Chain: E
Length: 32 nucleotides
Theoretical weight: 10.07 KDa
Source organism: Homo sapiens
Expression system: Not provided
PRDIII-I region of human interferon-B promoter strand 2 Chain: F
Molecule details ›
Chain: F
Length: 32 nucleotides
Theoretical weight: 9.61 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: C2
Unit cell:
a: 173.417Å b: 46.132Å c: 129.325Å
α: 90° β: 129.42° γ: 90°
R-values:
R R work R free
0.185 0.181 0.264
Expression systems:
  • Escherichia coli
  • Not provided