PDBe 2p75

X-ray diffraction
1.7Å resolution

Crystal structure of TTHB049 from Thermus thermophilus HB8

Released:
Source organism: Thermus thermophilus HB8
Entry authors: Sugahara M, Tanaka Y, Matsuura Y, Nakamoto T, Kunishima N, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed:
Adenosylcobalamin 5'-phosphate + H(2)O = coenzyme B12 + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-ribazole-5'-phosphate phosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 177 amino acids
Theoretical weight: 19.65 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q53WB3 (Residues: 1-177; Coverage: 100%)
Gene name: TTHB049
Sequence domains: Histidine phosphatase superfamily (branch 1)
Structure domains: Phosphoglycerate mutase-like

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: I222
Unit cell:
a: 83.65Å b: 91.632Å c: 112.099Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.198 0.217
Expression system: Escherichia coli BL21(DE3)