PDBe 2p4r

X-ray diffraction
2Å resolution

Structural basis for a novel interaction between AIP4 and beta-PIX


Function and Biology Details

Reaction catalysed:
(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Rho guanine nucleotide exchange factor 7 Chain: A
Molecule details ›
Chain: A
Length: 59 amino acids
Theoretical weight: 6.73 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
  • Canonical: O55043 (Residues: 10-63; Coverage: 8%)
Gene names: Arhgef7, Pak3bp, Pixb
Sequence domains: Variant SH3 domain
Structure domains: SH3 Domains
E3 ubiquitin-protein ligase Itchy homolog Chain: T
Molecule details ›
Chain: T
Length: 25 amino acids
Theoretical weight: 2.7 KDa
Source organism: Homo sapiens
Expression system: Not provided
  • Canonical: Q96J02 (Residues: 246-270; Coverage: 3%)
Gene name: ITCH

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P6122
Unit cell:
a: 41.747Å b: 41.747Å c: 149.278Å
α: 90° β: 90° γ: 120°
R R work R free
0.218 0.217 0.246
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided