PDBe 2ozl

X-ray diffraction
1.9Å resolution

Human pyruvate dehydrogenase S264E variant

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial Chains: A, C
Molecule details ›
Chains: A, C
Length: 365 amino acids
Theoretical weight: 40.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P08559 (Residues: 30-390; Coverage: 93%)
Gene names: PDHA1, PHE1A
Sequence domains: Dehydrogenase E1 component
Structure domains: Rossmann fold
Pyruvate dehydrogenase E1 component subunit beta, mitochondrial Chains: B, D
Molecule details ›
Chains: B, D
Length: 341 amino acids
Theoretical weight: 37.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P11177 (Residues: 32-359; Coverage: 91%)
Gene names: PDHB, PHE1B
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand TPP 2 x TPP
2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P212121
Unit cell:
a: 64.9Å b: 126.4Å c: 190.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.186 0.221
Expression system: Escherichia coli