PDBe 2ovg

X-ray diffraction
1.35Å resolution

Lambda Cro Q27P/A29S/K32Q triple mutant at 1.35 A in space group P3221

Released:

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Regulatory protein cro Chain: A
Molecule details ›
Chain: A
Length: 66 amino acids
Theoretical weight: 7.36 KDa
Source organism: Escherichia virus Lambda
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03040 (Residues: 1-66; Coverage: 100%)
Gene names: cro, lambdap57
Sequence domains: Cro
Structure domains: CRO Repressor

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: P3221
Unit cell:
a: 50.524Å b: 50.524Å c: 48.148Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.134 0.132 0.171
Expression system: Escherichia coli BL21(DE3)