PDBe 2o97

X-ray diffraction
2.45Å resolution

Crystal Structure of E. coli HU heterodimer

Released:
Source organism: Escherichia coli
Primary publication:
Spiral structure of Escherichia coli HUalphabeta provides foundation for DNA supercoiling.
Proc. Natl. Acad. Sci. U.S.A. 104 4309-14 (2007)
PMID: 17360520

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero octamer
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA-binding protein HU-alpha Chain: A
Molecule details ›
Chain: A
Length: 90 amino acids
Theoretical weight: 9.55 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ACF0 (Residues: 1-90; Coverage: 100%)
Gene names: JW3964, b4000, hupA
Sequence domains: Bacterial DNA-binding protein
Structure domains: IHF-like DNA-binding proteins
DNA-binding protein HU-beta Chain: B
Molecule details ›
Chain: B
Length: 90 amino acids
Theoretical weight: 9.24 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ACF4 (Residues: 1-90; Coverage: 100%)
Gene names: JW0430, b0440, hopD, hupB
Sequence domains: Bacterial DNA-binding protein
Structure domains: IHF-like DNA-binding proteins

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: I41
Unit cell:
a: 82.915Å b: 82.915Å c: 61.048Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.226 0.264
Expression system: Escherichia coli