PDBe 2o8c

X-ray diffraction
3.37Å resolution

human MutSalpha (MSH2/MSH6) bound to ADP and an O6-methyl-guanine T mispair

Released:
Source organism: Homo sapiens

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
DNA mismatch repair protein Msh2 Chain: A
Molecule details ›
Chain: A
Length: 934 amino acids
Theoretical weight: 104.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: NEW P43246 (Residues: 1-934; Coverage: 100%)
Gene name: MSH2
Sequence domains:
Structure domains:
DNA mismatch repair protein Msh6 Chain: B
Molecule details ›
Chain: B
Length: 1022 amino acids
Theoretical weight: 116.06 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: NEW P52701 (Residues: 341-1360; Coverage: 75%)
Gene names: GTBP, MSH6
Sequence domains:
Structure domains:
5'-D(*GP*AP*AP*CP*CP*GP*CP*(6OG)P*CP*GP*CP*TP*AP*GP*G)-3' Chain: E
Molecule details ›
Chain: E
Length: 15 nucleotides
Theoretical weight: 4.63 KDa
5'-D(*CP*CP*TP*AP*GP*CP*GP*TP*GP*CP*GP*GP*TP*TP*C)-3' Chain: F
Molecule details ›
Chain: F
Length: 15 nucleotides
Theoretical weight: 4.58 KDa

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P4332
Unit cell:
a: 259.81Å b: 259.81Å c: 259.81Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.258 0.256 0.29
Expression system: Spodoptera frugiperda