PDBe 2o61

X-ray diffraction
2.8Å resolution

Crystal Structure of NFkB, IRF7, IRF3 bound to the interferon-b enhancer

Released:
Source organism: Homo sapiens
Primary publication:
An atomic model of the interferon-beta enhanceosome.
Cell 129 1111-23 (2007)
PMID: 17574024

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Nuclear factor NF-kappa-B p50 subunit Chain: B
Molecule details ›
Chain: B
Length: 314 amino acids
Theoretical weight: 35.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P19838 (Residues: 40-352; Coverage: 32%)
Gene name: NFKB1
Sequence domains: Rel homology DNA-binding domain
Structure domains:
Transcription factor p65; Interferon regulatory factor 3; Interferon regulatory factor 7 Chain: A
Molecule details ›
Chain: A
Length: 540 amino acids
Theoretical weight: 61.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q04206 (Residues: 20-291; Coverage: 49%)
  • Canonical: Q92985 (Residues: 8-125; Coverage: 24%)
  • Canonical: Q14653 (Residues: 9-91, 92-111; Coverage: 24%)
  • nullnullnull
Gene names: IRF3, IRF7, NFKB3, RELA
Sequence domains:
Structure domains:
34-MER Chain: F
Molecule details ›
Chain: F
Length: 34 nucleotides
Theoretical weight: 10.26 KDa
36-MER Chain: E
Molecule details ›
Chain: E
Length: 36 nucleotides
Theoretical weight: 11.27 KDa

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P212121
Unit cell:
a: 95.222Å b: 116.37Å c: 134.237Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.245 0.245 0.278
Expression system: Escherichia coli