PDBe 2o4a

X-ray diffraction
1.75Å resolution

Crystal Structure of the N-terminal CUT Domain of SATB1 Bound to Matrix Attachment Region DNA

Released:

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA-binding protein SATB1 Chain: A
Molecule details ›
Chain: A
Length: 93 amino acids
Theoretical weight: 10.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: NEW Q01826 (Residues: 367-452; Coverage: 11%)
Gene name: SATB1
Structure domains: lambda repressor-like DNA-binding domains
DNA (5'-D(*DGP*DCP*DTP*DAP*DAP*DTP*DAP*DTP*DAP*DTP*DGP*DC)-3') Chain: B
Molecule details ›
Chain: B
Length: 12 nucleotides
Theoretical weight: 3.66 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*DGP*DCP*DAP*DTP*DAP*DTP*DAP*DTP*DTP*DAP*DGP*DC)-3') Chain: C
Molecule details ›
Chain: C
Length: 12 nucleotides
Theoretical weight: 3.66 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P1
Unit cell:
a: 32.659Å b: 38.318Å c: 40.709Å
α: 71.34° β: 82.5° γ: 66.63°
R-values:
R R work R free
0.245 0.245 0.282
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided