› 2o3f

X-ray diffraction
1.75Å resolution

Structural Genomics, the crystal structure of the N-terminal domain of the putative transcriptional regulator ybbH from Bacillus subtilis subsp. subtilis str. 168.

Released:
DOI: 10.2210/pdb2o3f/pdb
PDB entry 2o3f coloured by chain, front view.
PDB entry 2o3f coloured by chain, side view.
PDB entry 2o3f coloured by chain, top view.
Source organism: Bacillus subtilis
Entry authors: Tan K, Bigelow L, Abdullah J, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175059 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uncharacterized HTH-type transcriptional regulator YbbH Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 111 amino acids
Theoretical weight: 12.14 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q45581 (Residues: 1-108; Coverage: 38%)
Gene names: BSU01690, ybbH
Sequence domains: Helix-turn-helix domain, rpiR family
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

1 bound ligand:
Ligand SO4 15 x SO4
2 modified residues:
Ligand MSE 9 x MSE
Ligand MLY 15 x MLY

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P2
Unit cell:
a: 58.898Å b: 48.06Å c: 58.854Å
α: 90° β: 119.92° γ: 90°
R-values:
R R work R free
0.183 0.181 0.215
Expression system: Escherichia coli BL21

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