PDBe 2o02

X-ray diffraction
1.5Å resolution

Phosphorylation independent interactions between 14-3-3 and Exoenzyme S: from structure to pathogenesis

Released:

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein zeta/delta Chains: A, B
Molecule details ›
Chains: A, B
Length: 230 amino acids
Theoretical weight: 26.32 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63104 (Residues: 1-230; Coverage: 94%)
Gene name: YWHAZ
Sequence domains: 14-3-3 protein
Structure domains: 14-3-3 domain
ExoS (416-430) peptide Chains: P, Q
Molecule details ›
Chains: P, Q
Length: 14 amino acids
Theoretical weight: 1.37 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 71.33Å b: 72.238Å c: 125.664Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.151 0.147 0.212
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided