PDBe 2o01

X-ray diffraction
3.4Å resolution

The Structure of a plant photosystem I supercomplex at 3.4 Angstrom resolution

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 17-mer (preferred)
Entry contents:
17 distinct polypeptide molecules
Macromolecules (17 distinct):
Photosystem I P700 chlorophyll a apoprotein A1 Chain: A
Molecule details ›
Chain: A
Length: 754 amino acids
Theoretical weight: 83.67 KDa
Source organism: Pisum sativum
UniProt:
  • Canonical: P05310 (Residues: 5-758; Coverage: 100%)
Gene names: psaA, psaA1
Sequence domains: Photosystem I psaA/psaB protein
Structure domains: Photosystem I p700 chlorophyll A apoprotein A1
Photosystem I P700 chlorophyll a apoprotein A2 Chain: B
Molecule details ›
Chain: B
Length: 732 amino acids
Theoretical weight: 82.23 KDa
Source organism: Pisum sativum
UniProt:
  • Canonical: P05311 (Residues: 2-733; Coverage: 100%)
Gene names: psaA2, psaB
Sequence domains: Photosystem I psaA/psaB protein
Structure domains: Photosystem I PsaA/PsaB
Photosystem I iron-sulfur center Chain: C
Molecule details ›
Chain: C
Length: 80 amino acids
Theoretical weight: 8.86 KDa
Source organism: Pisum sativum
UniProt:
  • Canonical: P10793 (Residues: 2-81; Coverage: 99%)
Gene names: frxA, psaC
Sequence domains: 4Fe-4S dicluster domain
Structure domains: Alpha-Beta Plaits
Photosystem I reaction center subunit II, chloroplastic Chain: D
Molecule details ›
Chain: D
Length: 138 amino acids
Theoretical weight: 15.55 KDa
Source organism: Spinacia oleracea
UniProt:
  • Canonical: P12353 (Residues: 75-212; Coverage: 65%)
Gene name: psaD
Sequence domains: PsaD
Photosystem I reaction center subunit IV A, chloroplastic Chain: E
Molecule details ›
Chain: E
Length: 62 amino acids
Theoretical weight: 7.13 KDa
Source organism: Arabidopsis thaliana
UniProt:
  • Canonical: Q9S831 (Residues: 81-142; Coverage: 43%)
Gene names: At4g28750, F16A16.140, PSAE1
Sequence domains: Photosystem I reaction centre subunit IV / PsaE
Structure domains: SH3 type barrels.
Photosystem I reaction center subunit III, chloroplastic Chain: F
Molecule details ›
Chain: F
Length: 154 amino acids
Theoretical weight: 17.3 KDa
Source organism: Spinacia oleracea
UniProt:
  • Canonical: P12355 (Residues: 78-231; Coverage: 67%)
Gene name: PSAF
Structure domains: Photosystem I PsaF, reaction centre subunit III
Photosystem I reaction center subunit V, chloroplastic Chain: G
Molecule details ›
Chain: G
Length: 95 amino acids
Theoretical weight: 10.52 KDa
Source organism: Spinacia oleracea
UniProt:
  • Canonical: P12357 (Residues: 73-167; Coverage: 57%)
Gene name: PSAG
Structure domains: Chlorophyll a-b binding protein like
Photosystem I reaction center subunit VI, chloroplastic Chain: H
Molecule details ›
Chain: H
Length: 75 amino acids
Theoretical weight: 8.14 KDa
Source organism: Spinacia oleracea
UniProt:
  • Canonical: P22179 (Residues: 70-144; Coverage: 52%)
Gene name: PSAH
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Photosystem I reaction center subunit VIII Chain: I
Molecule details ›
Chain: I
Length: 30 amino acids
Theoretical weight: 3.27 KDa
Source organism: Pisum sativum
UniProt:
  • Canonical: P17227 (Residues: 1-30; Coverage: 75%)
Gene name: psaI
Sequence domains: Photosystem I reaction centre subunit VIII
Photosystem I reaction center subunit IX Chain: J
Molecule details ›
Chain: J
Length: 42 amino acids
Theoretical weight: 4.85 KDa
Source organism: Spinacia oleracea
UniProt:
  • Canonical: P17230 (Residues: 1-42; Coverage: 96%)
Gene name: psaJ
Sequence domains: Photosystem I reaction centre subunit IX / PsaJ
Structure domains: Single helix bin
Photosystem I reaction center subunit psaK, chloroplast Chain: K
Molecule details ›
Chain: K
Length: 38 amino acids
Theoretical weight: 3.25 KDa
Source organism: Arabidopsis thaliana
Photosystem I reaction center subunit XI, chloroplastic Chain: L
Molecule details ›
Chain: L
Length: 164 amino acids
Theoretical weight: 17.48 KDa
Source organism: Spinacia oleracea
UniProt:
  • Canonical: Q41385 (Residues: 53-216; Coverage: 76%)
Gene name: PSAL
Sequence domains: Photosystem I reaction centre subunit XI
Structure domains: Photosystem I PsaL, reaction centre subunit XI
Photosystem I-N subunit Chain: N
Molecule details ›
Chain: N
Length: 85 amino acids
Theoretical weight: 9.77 KDa
Source organism: Phaseolus vulgaris
UniProt:
  • Canonical: Q84U30 (Residues: 86-170; Coverage: 50%)
Gene names: PHAVU_002G269400g, PSI-N
Structure domains: Single helix bin
Chlorophyll a-b binding protein 6, chloroplastic Chain: 1
Molecule details ›
Chain: 1
Length: 187 amino acids
Theoretical weight: 20.42 KDa
Source organism: Arabidopsis thaliana
UniProt:
  • Canonical: Q01667 (Residues: 49-235; Coverage: 78%)
Gene names: At3g54890, CAB6, F28P10.130, LHCA1
Sequence domains: Chlorophyll A-B binding protein
Structure domains: Chlorophyll a/b binding protein domain
Chlorophyll a-b binding protein, chloroplastic Chain: 2
Molecule details ›
Chain: 2
Length: 186 amino acids
Theoretical weight: 20.58 KDa
Source organism: Pisum sativum
UniProt:
  • Canonical: Q41038 (Residues: 74-259; Coverage: 69%)
Gene name: lhaB
Structure domains: Chlorophyll a/b binding protein domain
Photosystem I chlorophyll a/b-binding protein 3-1, chloroplastic Chain: 3
Molecule details ›
Chain: 3
Length: 165 amino acids
Theoretical weight: 17.93 KDa
Source organism: Arabidopsis thaliana
UniProt:
  • Canonical: Q9SY97 (Residues: 95-259; Coverage: 60%)
Gene names: At1g61520, LHCA3, T25B24.12
Structure domains: Chlorophyll a/b binding protein domain
Chlorophyll a-b binding protein P4, chloroplastic Chain: 4
Molecule details ›
Chain: 4
Length: 165 amino acids
Theoretical weight: 18.56 KDa
Source organism: Pisum sativum
UniProt:
  • Canonical: Q9SQL2 (Residues: 85-249; Coverage: 66%)
Gene name: lhcA-P4
Structure domains: Chlorophyll a/b binding protein domain

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P21
Unit cell:
a: 120.054Å b: 188.784Å c: 127.518Å
α: 90° β: 95.19° γ: 90°
R-values:
R R work R free
0.351 0.348 0.409