PDBe 2nz8

X-ray diffraction
2Å resolution

N-terminal DHPH cassette of Trio in complex with nucleotide-free Rac1

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ras-related C3 botulinum toxin substrate 1 Chain: A
Molecule details ›
Chain: A
Length: 177 amino acids
Theoretical weight: 19.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63000 (Residues: 1-177; Coverage: 92%)
Gene names: MIG5, RAC1, TC25
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Triple functional domain protein Chain: B
Molecule details ›
Chain: B
Length: 313 amino acids
Theoretical weight: 36.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O75962 (Residues: 1285-1594; Coverage: 10%)
Gene name: TRIO
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21212
Unit cell:
a: 97.492Å b: 108.558Å c: 53.416Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.223 0.249
Expression system: Escherichia coli BL21(DE3)