PDBe 2ny6

X-ray diffraction
2.8Å resolution

HIV-1 gp120 Envelope Glycoprotein (M95W, W96C, I109C, T123C, T257S, V275C,S334A, S375W, Q428C, G431C) Complexed with CD4 and Antibody 17b

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Envelope glycoprotein Chain: A
Molecule details ›
Chain: A
Length: 317 amino acids
Theoretical weight: 35.11 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8Q7Z9 (Residues: 71-173; Coverage: 50%)
Gene name: env
Structure domains: Human immunodeficiency virus 1, Gp160, envelope glycoprotein
T-cell surface glycoprotein CD4 Chain: B
Molecule details ›
Chain: B
Length: 184 amino acids
Theoretical weight: 20.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01730 (Residues: 26-208; Coverage: 42%)
Gene name: CD4
Sequence domains: Immunoglobulin C2-set domain
Structure domains: Immunoglobulins
IGK@ protein Chain: C
Molecule details ›
Chain: C
Length: 214 amino acids
Theoretical weight: 23.4 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q6P5S8 (Residues: 21-114, 115-234; Coverage: 99%)
Gene name: IGK@
Sequence domains:
Structure domains: Immunoglobulins
ANTIBODY 17B, HEAVY CHAIN Chain: D
Molecule details ›
Chain: D
Length: 229 amino acids
Theoretical weight: 24.46 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q6N030 (Residues: 20-117, 118-123, 124-242; Coverage: 45%)
Gene name: DKFZp686I15212
Sequence domains:
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P2221
Unit cell:
a: 72.31Å b: 87.018Å c: 196.078Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.192 0.276
Expression systems:
  • Homo sapiens
  • Escherichia coli