PDBe 2nxy

X-ray diffraction
2Å resolution

HIV-1 gp120 Envelope Glycoprotein(S334A) Complexed with CD4 and Antibody 17b

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Envelope glycoprotein Chain: A
Molecule details ›
Chain: A
Length: 317 amino acids
Theoretical weight: 35.04 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
UniProt:
  • Canonical: NEW Q49DS8 (Residues: 1-80, 81-119, 120-125, 126-205; Coverage: 86%)
Gene name: env
Structure domains: Human immunodeficiency virus 1, Gp160, envelope glycoprotein
T-cell surface glycoprotein CD4 Chain: B
Molecule details ›
Chain: B
Length: 184 amino acids
Theoretical weight: 20.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: NEW P01730 (Residues: 26-208; Coverage: 42%)
Gene name: CD4
Sequence domains: Immunoglobulin C2-set domain
Structure domains: Immunoglobulins
IGK@ protein Chain: C
Molecule details ›
Chain: C
Length: 214 amino acids
Theoretical weight: 23.4 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: NEW Q6P5S8 (Residues: 21-114, 115-234; Coverage: 99%)
Gene name: IGK@
Sequence domains:
Structure domains: Immunoglobulins
ANTIBODY 17B, HEAVY CHAIN Chain: D
Molecule details ›
Chain: D
Length: 229 amino acids
Theoretical weight: 24.46 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: NEW Q6N095 (Residues: 20-131, 132-246; Coverage: 50%)
Gene name: DKFZp686K03196
Sequence domains:
Structure domains: Immunoglobulins

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P2221
Unit cell:
a: 71.346Å b: 88.095Å c: 195.981Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.181 0.231
Expression systems:
  • Homo sapiens
  • Escherichia coli