PDBe 2nx5

X-ray diffraction
2.7Å resolution

Crystal structure of ELS4 TCR bound to HLA-B*3501 presenting EBV peptide EPLPQGQLTAY at 1.7A

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
HLA class I histocompatibility antigen, B-35 alpha chain Chains: A, F, K, Q
Molecule details ›
Chains: A, F, K, Q
Length: 276 amino acids
Theoretical weight: 31.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P30685 (Residues: 25-300; Coverage: 82%)
Gene names: HLA-B, HLAB
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, G, L, R
Molecule details ›
Chains: B, G, L, R
Length: 99 amino acids
Theoretical weight: 11.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Trans-activator protein BZLF1 Chains: C, H, M, S
Molecule details ›
Chains: C, H, M, S
Length: 11 amino acids
Theoretical weight: 1.22 KDa
Source organism: Human herpesvirus 4 strain B95-8
Expression system: Not provided
UniProt:
  • Canonical: P03206 (Residues: 54-64; Coverage: 5%)
Gene name: BZLF1
T cell receptor alpha constant Chains: D, I, N, T
Molecule details ›
Chains: D, I, N, T
Length: 188 amino acids
Theoretical weight: 20.89 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01848 (Residues: 1-77; Coverage: 55%)
Gene names: TCRA, TRAC
Structure domains: Immunoglobulins
T cell receptor beta constant 1 Chains: E, J, P, U
Molecule details ›
Chains: E, J, P, U
Length: 243 amino acids
Theoretical weight: 27.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01850 (Residues: 1-129; Coverage: 73%)
Gene name: TRBC1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P21
Unit cell:
a: 118.479Å b: 118.067Å c: 131.465Å
α: 90° β: 96.04° γ: 90°
R-values:
R R work R free
0.272 0.269 0.327
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided