PDBe 2nut

X-ray diffraction
2.3Å resolution

Crystal Structure of the human Sec23a/24a heterodimer, complexed with the SNARE protein Sec22b

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Protein transport protein Sec23A Chain: A
Molecule details ›
Chain: A
Length: 769 amino acids
Theoretical weight: 86.55 KDa
Source organism: Homo sapiens
Expression system: Hi5 insect cells
UniProt:
  • Canonical: NEW Q15436 (Residues: 1-765; Coverage: 100%)
Gene name: SEC23A
Sequence domains:
Structure domains:
Protein transport protein Sec24A Chain: B
Molecule details ›
Chain: B
Length: 753 amino acids
Theoretical weight: 84.96 KDa
Source organism: Homo sapiens
Expression system: Hi5 insect cells
UniProt:
  • Canonical: NEW O95486 (Residues: 341-1093; Coverage: 69%)
Gene name: SEC24A
Sequence domains:
Structure domains:
Vesicle-trafficking protein SEC22b Chain: C
Molecule details ›
Chain: C
Length: 196 amino acids
Theoretical weight: 22.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: NEW O75396 (Residues: 1-195; Coverage: 91%)
Gene names: SEC22B, SEC22L1
Sequence domains: Regulated-SNARE-like domain
Structure domains: Beta-Lactamase

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: C2
Unit cell:
a: 148.28Å b: 97.58Å c: 129.8Å
α: 90° β: 90.15° γ: 90°
R-values:
R R work R free
0.219 0.219 0.268
Expression systems:
  • Hi5 insect cells
  • Escherichia coli