› 2ntc

X-ray diffraction
2.4Å resolution

Crystal Structure of sv40 large T antigen origin binding domain with DNA

Released:
DOI: 10.2210/pdb2ntc/pdb
PDB entry 2ntc coloured by chain, front view.
PDB entry 2ntc coloured by chain, side view.
PDB entry 2ntc coloured by chain, top view.
Source organisms:
Primary publication:
The crystal structure of the SV40 T-antigen origin binding domain in complex with DNA.
Meinke G, Phelan P, Moine S, Bochkareva E, Bochkarev A, Bullock PA, Bohm A
PLoS Biol 5 e23 (2007)
PMID: 17253903

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115687 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Large T antigen Chains: A, B
Molecule details ›
Chains: A, B
Length: 134 amino acids
Theoretical weight: 15.41 KDa
Source organism: Macaca mulatta polyomavirus 1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q98ZP7 (Residues: 131-260; Coverage: 24%)
Sequence domains: Origin of replication binding protein
Structure domains: Replication Protein E1; Chain: A,
21-nt PEN element of the SV40 DNA origin Chain: W
Molecule details ›
Chain: W
Length: 21 nucleotides
Theoretical weight: 6.42 KDa
Source organism: Synthetic construct
Expression system: Not provided
21-nt PEN element of the SV40 DNA origin Chain: C
Molecule details ›
Chain: C
Length: 21 nucleotides
Theoretical weight: 6.47 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 64.489Å b: 68.341Å c: 97.262Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.205 0.287
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

11 review citations

The Mcm complex: unwinding the mechanism of a replicative helicase.
Bochman et al. (2009)
10 more

11 mentions without citation

The critical protein interactions and structures that elicit growth deregulation in cancer and viral replication.
Ou et al. (2011)
10 more