PDBe 2n7k

Solution NMR

Unveiling the structural determinants of KIAA0323 binding preference for NEDD8

Released:
Source organism: Homo sapiens
Entry authors: Santonico E, Nepravishta R, Mattioni A, Valentini E, Mandaliti W, Procopio R, Iannuccelli M, Castagnoli L, Polo S, Paci M, Cesareni G

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
NEDD8 Chain: A
Molecule details ›
Chain: A
Length: 81 amino acids
Theoretical weight: 9.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15843 (Residues: 1-81; Coverage: 100%)
Gene name: NEDD8
Sequence domains: Ubiquitin family
Protein KHNYN Chain: B
Molecule details ›
Chain: B
Length: 52 amino acids
Theoretical weight: 6.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O15037 (Residues: 627-678; Coverage: 8%)
Gene names: KHNYN, KIAA0323

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 14%
Refinement method: simulated annealing
Chemical shifts: BMR25808  
Expression system: Escherichia coli